Oligo & Antibody Molarity Calculator

This oligo and antibody molarity calculator converts the concentration of oligonucleotides or antibodies from mass units (mg/mL, µg/mL) to molar concentration (µM, nM). Enter concentration and molecular weight for instant results.

Formula

M = concentration / MW

1 Concentration

2 Molecular Weight

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Concentration

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Molecular Weight

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Molarity

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How It Works

Concentration

Enter value

Molecular Weight

Enter value

Molarity

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What is Oligo & Antibody Molarity?

Oligo and antibody molarity expresses the molar concentration of these large biomolecules. Oligonucleotides (short DNA/RNA, 15–60 nt) typically have MW of 5–20 kDa. Antibodies (IgG) have MW of ~150 kDa.

For oligonucleotides, MW ≈ (number of bases × 330) − 62 for ssDNA. For antibodies, IgG ≈ 150 kDa, IgM ≈ 970 kDa, Fab fragments ≈ 50 kDa.

Molar concentration is needed for hybridization reactions (oligos), ELISA/Western blot antibody dilutions, and any experiment requiring stoichiometric ratios of these biomolecules.

Molarity = concentration (mg/mL) ÷ molecular weight (g/mol)

Visual Concept

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Chemical Solution

Concentration measured in moles per liter (mol/L)

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Unit: M (Molar)

1 M = 1 mol of solute per 1 liter of solution

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Key Relationship

Molarity = Moles of Solute ÷ Volume of Solution

Oligo & Antibody Molarity Formula

The formula for calculating molarity from oligo/antibody concentration:

molarity = moles of solute ÷ volume of solution (L)

To use this formula, you need concentration and molecular weight. The calculator handles all unit conversions automatically.

Enter your values below to see the oligo & antibody molarity formula in action.

mass ÷ volume = concentration = molarity × molar mass

Where mass is in grams (g), volume is in liters (L), and molar mass is in g/mol.

Formula Breakdown

M = n / V

Molarity (mol/L)

Moles of acid (mol)

Volume of solution (L)

💡 For polyprotic acids, multiply by the number of H⁺ ions

How to Calculate Oligo & Antibody Molarity

Follow these steps to calculate molarity from oligo/antibody concentration:

Identify the oligo/antibody concentration of your solute and gather all known values.
Find the molar mass of the substance from the periodic table or a chemical database.
Enter your oligo/antibody concentration value and select the appropriate unit from the dropdown.
Enter the volume of the solution in liters (L) or select another volume unit.
Click Calculate to get the molarity result instantly.
Verify the result using the step-by-step breakdown provided by the calculator.

Step-by-Step Process

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Identify the Acid

Determine the acid compound and its molecular formula

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Measure Mass

Weigh the acid solute in grams (g)

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Find Molar Mass

Look up the molar mass (g/mol) from periodic table

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Calculate Moles

Divide mass by molar mass: n = m / MW

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Measure Volume

Measure solution volume in liters (L)

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Compute Molarity

M = moles ÷ volume (mol/L)

Oligo & Antibody Molarity Calculator Examples

The examples below show how to use the oligo & antibody molarity calculator in practice.

Example 1: What is the molarity of 1 mg/mL IgG antibody? IgG MW ≈ 150 kDa = 150,000 g/mol. M = 1/150000 = 6.67 × 10⁻⁶ M = 6.67 µM.

Example 2: Using the formula: molarity = moles ÷ volume, compute the result and verify with the calculator above.

Example 3: Enter your own values into the calculator to see personalized step-by-step results.

Worked Example Example: HCl Solution

Mass 36.5 g

Molar Mass 36.46 g/mol

Volume 1 L

Molarity 1.00 M

Molarity Calculator Tools

Explore our collection of 20 specialized molarity calculators. Each tool is designed for a specific type of calculation with its own unique input fields and formulas.

FAQs

How do you calculate oligonucleotide molarity?

M = concentration (OD₂₆₀ units / mL × extinction coefficient correction) or simply mass (g/L) ÷ MW (g/mol).

What is the molarity of 1 mg/mL IgG antibody?

IgG MW ≈ 150 kDa = 150,000 g/mol. M = 1/150000 = 6.67 × 10⁻⁶ M = 6.67 µM.

How do I find oligo molecular weight?

Sum the molecular weights of each nucleotide. A rough estimate: MW ≈ (number of bases × 330) − 61.96 for ssDNA oligos.

What units does the Oligo & Antibody Molarity Calculator support?

The oligo & antibody molarity calculator supports mass units (g, mg, µg, kg), volume units (L, mL, µL, gal), molar mass (g/mol, kg/mol), and molarity units (M, mM, µM, nM, pM, fM, aM).

Is the Oligo & Antibody Molarity Calculator free to use?

Yes. The oligo & antibody molarity calculator is 100% free, requires no sign-up, and works on all devices. All calculations are performed instantly in your browser.