Reconstitution Molarity Calculator
This reconstitution molarity calculator calculates the volume of solvent needed to dissolve a lyophilized (freeze-dried) compound to achieve a target molar concentration. Enter the mass of compound, its molecular weight, and your target molarity to get the required reconstitution volume.
Reconstitution Molarity Calculator
Mass → Volume needed
Volume = mass / (MW × target M) What is Reconstitution Molarity?
Reconstitution is the process of dissolving a dry (lyophilized) compound in a solvent to create a solution of known concentration. This calculator finds the exact volume of solvent to add.
The formula rearranges the standard molarity equation: Volume = mass ÷ (MW × target molarity). The result tells you how much DMSO, water, or buffer to add to your vial.
Reconstitution calculations are critical for preparing drug stock solutions, reconstituting lyophilized proteins, peptides, and antibodies, and setting up bioassay compound plates.
Reconstitution Molarity Formula
The formula for calculating molarity from reconstitution:
To use this formula, you need mass of compound, MW, and target molarity. The calculator handles all unit conversions automatically.
Enter your values below to see the reconstitution molarity formula in action.
Where mass is in grams (g), volume is in liters (L), and molar mass is in g/mol.
How to Calculate Reconstitution Molarity
Follow these steps to calculate molarity from reconstitution:
- Identify the reconstitution of your solute and gather all known values.
- Find the molar mass of the substance from the periodic table or a chemical database.
- Enter your reconstitution value and select the appropriate unit from the dropdown.
- Enter the volume of the solution in liters (L) or select another volume unit.
- Click Calculate to get the molarity result instantly.
- Verify the result using the step-by-step breakdown provided by the calculator.
Reconstitution Molarity Calculator Examples
The examples below show how to use the reconstitution molarity calculator in practice.
Example 1: I have 1 mg of a 500 Da compound. How much solvent for 10 mM? V = 0.001 / (500 × 0.01) = 0.0002 L = 200 µL.
Example 2: Using the formula: molarity = moles ÷ volume, compute the result and verify with the calculator above.
Example 3: Enter your own values into the calculator to see personalized step-by-step results.
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FAQs
How do you calculate reconstitution volume?
Volume (L) = mass (g) / [MW (g/mol) × target molarity (M)]. Convert to mL or µL as needed.
I have 1 mg of a 500 Da compound. How much solvent for 10 mM?
V = 0.001 / (500 × 0.01) = 0.0002 L = 200 µL.
What solvent should I use?
Common solvents: DMSO for hydrophobic compounds, water or buffer for hydrophilic compounds. Check the compound datasheet.
What units does the Reconstitution Molarity Calculator support?
The reconstitution molarity calculator supports mass units (g, mg, µg, kg), volume units (L, mL, µL, gal), molar mass (g/mol, kg/mol), and molarity units (M, mM, µM, nM, pM, fM, aM).
Is the Reconstitution Molarity Calculator free to use?
Yes. The reconstitution molarity calculator is 100% free, requires no sign-up, and works on all devices. All calculations are performed instantly in your browser.